蛋白質研究和抗體純化用磁珠
為了能夠快速有效地純化抗體和標簽蛋白，金斯瑞（GenScript）提供了超順磁性的MagBeads系列磁珠。GenScript MagBeads偶聯有抗標簽抗體、重組蛋白、多肽和小分子金屬螯合試劑配基，可與抗體和帶標簽的蛋白以及Biotin標簽分子特異性地結合。
磁株分離純化方法可直接從細胞裂解物或細胞上清中純化目的抗體和蛋白，減少了離心和過濾步驟，提高了純化效率。高結合能力，高結合特異性和超快的磁響應特性，使得金斯瑞磁珠純化產品非常適合快速純化和高效濃縮目標產品，得到高得率和高純度的純化樣品。
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應用領域
- 抗體純化
- 標簽蛋白純化，如：His，GST，Flag和生物素等
- 高通量抗體和標簽蛋白的純化
Protein A、Protein G和Protein A/G 磁珠
標簽蛋白純化磁珠
抗體磁珠純化相關溶液
| 產品名稱 | 產品編號 | 規格 | 訂購 |
|---|---|---|---|
| AmMag? Wash buffer for antibody purification | B00045 | 210g/包*，干粉 | 詢價 |
*每包干粉可以配置成2 L緩沖溶液。
| AmMag? Protein A Magnetic beads 動態載量 (L00695) | ||||
| Sample concentration (hIgG) | RT / 2 H | 37°C / Overnight | 4°C / 2 H | 4°C / Overnight |
|---|---|---|---|---|
| 0.01mg/ml | 2.5mg/ml | 5 | 2 | 2.5mg/ml |
| 0.1mg/ml | 20mg/ml | 30mg/ml | 15 | 20 |
| 0.5mg/ml | 35mg/ml | 60mg/ml | 30mg/ml | 50mg/ml |
| 1mg/ml | 45mg/ml | 70mg/ml | 40 | 60 |
| 3mg/ml | 70mg/ml | 100mg/ml | 60 | 85 |
| Ni charged Magbeads 動態載量 (L00295) | ||||
| Sample concentration (MBP) | RT / 2 H | 37C / Overnight | 4C / 2 H | 4C / Overnight |
|---|---|---|---|---|
| 0.01mg/ml | 2.5mg/ml | 2.5mg/ml | ||
| 0.1mg/ml | 30mg/ml | 20mg/ml | 10mg/ml | 20mg/ml |
| 0.5mg/ml | 50mg/ml | 40mg/ml | 30mg/ml | 40mg/ml |
| 2mg/ml | 60mg/ml | 50mg/ml | 40mg/ml | 50mg/ml |
| AmMag?Ni Magnetic Beads動態載量 (L00776) | |
| Sample concentration (MBP) | RT / 1 H |
|---|---|
| 0.01mg/ml | 1-2mg/ml |
| 0.1mg/ml | 8mg/ml |
| 0.5mg/ml | 9mg/ml |
| 1mg/ml | 9mg/ml |
| 應用 | 產品名稱 | 原理 | 結合能力 | 規格 | 產品編號 |
|---|---|---|---|---|---|
| 抗體純化 | Protein A 磁珠 | 通過重組蛋白A或G，與不同物種IgG及其亞基的結合特性 | 10mg兔 IgG/ml磁珠 | 4ml | L00273 |
| Protein A MagBeads MX 磁珠 | 30mg人 IgG/ml磁珠 | 4ml | L00672 | ||
| AmMag™ 耐堿Protein A 磁珠 | 30mg人 IgG/ml磁珠 | 4ml | L00695 | ||
| Protein G 磁珠 | 10mg山羊 IgG/ml磁珠 | 2ml | L00274 | ||
| Protein G MagBeads MX 磁珠 | 25mg人 IgG/ml磁珠 | 4ml | L00673 | ||
| Protein A/G 磁珠 | 10mg山羊或兔 IgG/ml磁珠 | 2ml | L00277 | ||
| Protein A/G Magbeads MX 磁珠 | 30mg 人lgG/ml磁珠 | 2ml | L00894 | ||
| His標簽蛋白純化 | Ni-charged 磁珠 | 通過Ni2+捕獲His標簽蛋白 | 40mg 6×His標簽蛋白 (27kD)/ml磁珠 | 8ml | L00295 |
| AmMag™ Ni Magnetic beads | 通過帶鎳電荷的TED配體捕獲His標簽蛋白，耐EDTA和DTT | 10mg 6×His標簽蛋白 (27kD)/ml磁珠 | 4/40/400ml | L00776 | |
| Flag標簽蛋白純化 | MonoRab™ Anti-DYKDDDDK Magnetic Beads | 通過DYKDDDDK兔單抗捕獲Flag標簽蛋白 | 1.5mg DYKDDDDK標簽蛋白（50KDa）/ml磁珠 | 1/5/25ml | L00835 |
| GST融合蛋白純化 | GST磁珠 | 通過還原型谷胱甘肽捕獲GST融合蛋白 | 20 - 30mg GST/ml磁珠 | 8ml | L00895 |
| 生物素化樣品分離純化 | Streptavidin 磁珠 | 通過Streptavidin捕獲生物素化的樣品 | > 60nmol游離生物素/ml磁珠 | 2ml | L00936 |
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