MonoRab™ 兔單克隆抗體
兔單克隆抗體（mAbs）憑借其親和力高、特異性強、及識別抗原表位更豐富等獨特優勢，在基礎科研、診斷和醫療等領域都得到廣泛的應用。GenScript是全球第二家利用自主知識產權的雜交瘤細胞技術開發兔單克隆抗體的公司。
我們采用基于雜交瘤細胞技術的MonoRab™技術平臺，通過篩選獲得動物自身親和力成熟的特異性抗體，開發出性能優越的兔單克隆抗體。該平臺在以小分子藥、生物藥、甚至小鼠體內的非免疫性抗原等多種靶點的抗體開發中，具有非常巨大的技術優勢。
MonoRab™ Platform VS. Mouse Monoclonal Technology
| Comparison | MonoRab™ platform | Mouse mAb technology |
|---|---|---|
| Antigen Recognization | Capable of recognizing a variety of difficult antigens:
|
Limited immune response |
| Antibody Sensitivity | High It enables the detection of less abundant antigens with a minimum amount of antibody, making the MonoRab™ antibody a cost-effective reagent. |
Medium-High |
| Antibody Specificity | High This reduces background signals and allow for the precise targeting of desired antigens |
Medium-High |
| Antibody Affinity | Picomolar KD values This allows for the preservation of antibody-antigen interaction for a robust detection and function |
Nano or micromolar KD values |
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- Lane 1: MonoRab™ Anti-His antibody (4 μg) + His tagged fusion protein
- Lane 2: Rabbit IgG (4 μg) + His tagged fusion protein
- Lane 3: His tagged fusion protein (Input)
Western blot analysis of immunoprecipitation from cell lysates containing His fusion protein by using MonoRab™ Anti-His Tag (C-term) Antibody (25B6E11), mAb, Rabbit ( A01857-40)
Comparison of IHC using beta actin rabbit mAb on monkey heart tissue, 0.25 μg/ml, and hematoxylin counterstain.
GenScript MonoRab™ Beta Actin Antibody, mAb, Rabbit ( A01865-40) shows stronger signal with low background compared with other suppliers.
MonoRab™ Anti-Bevacizumab Antibody (30E1), mAb, Rabbit ( A01895-40) binds with Bevacizumab. While the antibody does not recognize the human IgG Fc fragment (data not shown).
EC50= 7.280 ng/ml
MonoRab™ Anti-Bevacizumab Antibody (46E3) [Biotin], mAb, Rabbit ( A01896-40) blocking with Vascular Endothelial Growth Factor 165 (VEGF 165).
EC50= 46.16 ng/ml
Standard curve of Bevacizumab Sandwich ELISA. It is developed by using MonoRab™ Anti-Bevacizumab Antibody (30E1), mAb, Rabbit ( A01895-40) and MonoRab™ Anti-Bevacizumab Antibody (46E3) [Biotin], mAb, Rabbit ( A01896-40) as the capture and detection antibodies, respectively.
The sensitivity of detecting Bevacizumab is up to 20 pg/ml.
MonoRab™ Ractopamine Antibody (A6), mAb, Rabbit ( A01872-40) binds with Ractopamine.
IC50= 51.37 pg/ml.
