ToxinEraser 內毒素去除系統

• 高結合能力，至少2, 000, 000EU/ml （柱體積）
• 高再生能力（>90%）
• 高穩定性和高去除力：可將樣品中內毒素含量降至0.1EU/ml^*

^*重復使用ToxinEraser 內毒素去除樹脂，最終去除效率根據樣品不同會有所區別。

金斯瑞ToxinEraser 是一種有效的內毒素去除工具，在重復使用之后可以將樣品中內毒素水平降低至小于1EU/ mg。該試劑盒可以用于去除蛋白質、多肽、抗體或DNA樣品中的內毒素。

產品訂購信息

相關產品

選擇指導

1. 怎樣為醫療器械檢測內毒素？

   為醫療器械管腔注入不含內毒素的水15毫升（如輸液裝置、透析管等），密封兩端后在37°C水浴孵育2小時，然后將水轉移至無內毒素的小瓶。使用ToxinSensor 鱟試劑檢測法試劑盒（目錄號L00350）檢測水體中的內毒素濃度，在水中的總內毒素值便可以確定。

2. 制備標準品溶液時能否使用塑料容器？

   一般而言，內毒素在塑料表面的附著比玻璃表面更強，標準的實驗室壓力蒸汽滅菌對內毒素幾乎不會有任何影響。如要使用玻璃器皿，建議在180°C過夜滅菌以破壞任何附加的內毒素分子。我們建議您僅使用新的塑料制品，并在準備標準品溶液時確認無內毒素。金斯瑞提供Toxinsensor無內毒素離心管（目錄號M01072）用于樣品處理。

3. 標準品溶液能否重復使用？

   標準品的母液是可以保存后使用的，但是不可以反復凍融。母液的保存期限請參考說明書。而不同濃度標準品的梯度稀釋液是不可以保存后使用的，因為濃度太低，效價會降低，會影響線性。所以，標準品的稀釋液建議每次實驗時新鮮制備。

4. 如何確定最終的數據的可靠性？

   首先，建議制備所需實驗試劑時在通風櫥內進行以避免污染；
   對于鱟試劑檢測法試劑盒（目錄號L00350），如果樣品的OD值在標準范圍內，最終的數據可以獲得良好的線性（R≥0.98）；
   對于凝膠檢測法試劑盒（目錄號L00351），可以通過陰性和陽性對照，獲得可靠的結果。如果在陰性對照下得到一個陽性的結果，這表明，鱟試劑的水可能已經被污染；如果陽性對照不凝膠，需要考慮標準品的渦旋和稀釋操作以及孵育溫度是否符合要求，也可能是鱟試劑保存不當，已經失去活性。

5. 是否可以使用96孔板讀取545nm的吸光值？

   鱟試劑檢測法試劑盒（L00350）為高靈敏度定量設計，不適用于96孔板；但是可以在離心管中進行實驗，在顯色后將溶液轉移至96孔板中進行讀取。

了解更多 >>

• Bergenfelz C., et al. Wnt5a inhibits human monocyte derived myeloid dendritic cell generation. Scand J Immunol. 2013 Aug;78(2):194-204.
• Shalaby KH., et al. Inhaled Birch Pollen Extract Induces Airway Hyperresponsiveness via Oxidative Stress but Independently of Pollen-Intrinsic NADPH Oxidase Activity, or the TLR4-TRIF Pathway. J Immunol. 2013 Jul 15;191(2):922-33.
• Clark KD., et al. Hemolymph melanization in the silkmoth Bombyx mori involves formation of a high molecular mass complex that metabolizes tyrosine. J Biol Chem. 2013 May 17;288(20):14476-87.
• Müller M., et al. Alterations in the secretory pattern of dermal dendritic cells following melanin uptake. Cell Tissue Res. 2013 Jun;352(3):599-610.
• Kim EH., et al. Prokaryote-expressed M2e protein improves H9N2 influenza vaccine efficacy and protection against lethal influenza a virus in mice. Virol J. 2013 Apr 3;10:104.
• O'Meara CP., et al. Immunization with a MOMP-Based Vaccine Protects Mice against a Pulmonary Chlamydia Challenge and Identifies a Disconnection between Infection and Pathology. PLoS One. 2013 Apr 16;8(4):e61962.
• Kovacs-Nolan J., et al. β-1,4-mannobiose stimulates innate immune responses and induces TLR4-dependent activation of mouse macrophages but reduces severity of inflammation during endotoxemia in mice. J Nutr. 2013 Mar;143(3):384-91.
• Schepetkin IA., et al. Immunomodulatory and hemagglutinating activities of acidic polysaccharides isolated from Combretum racemosum. Int Immunopharmacol. 2013 Mar;15(3):628-37.
• Ambalavanan N., et al. Titanium oxide nanoparticle instillation induces inflammation and inhibits lung development in mice. Am J Physiol Lung Cell Mol Physiol. 2013 Feb 1;304(3):L152-61.
• Mishra B., et al. A novel antimicrobial peptide derived from modified N-terminal domain of bovine lactoferrin: design, synthesis, activity against multidrug -resistant bacteria and Candida. Biochim Biophys Acta. 2013 Feb;1828(2):677-86.
• A Mountney, et al. Sialidase, Chondroitinase Abc And Combination Therapy After Spinal Cord Contusion Injury. J Neurotrauma. 2013 Feb 1;30(3):181-90.
• Bastiaan-Net S., et al. Biochemical And Functional Characterization Of Recombinant Fungal Immunomodulatory Proteins (Rfips). Int Immunopharmacol. 2013 Jan;15(1):167-75.
• Sameera Sayeed, et al. Multifunctional Role Of Human Splunc1 In Pseudomonas Aeruginosa Infection. Infect Immun. 2013 Jan;81(1):285-91.
• K Kouakou, et al. Immunomodulatory activity of polysaccharides isolated from Alchornea cordifolia. J Ethnopharmacol. 2013 Mar 7;146(1):232-42.
• Kaliannan K., et al. Intestinal alkaline phosphatase prevents metabolic syndrome in mice. Proc Natl Acad Sci USA. 2013 Apr 23;110(17):7003-8.
• Gomez G., et al. Immunogenic and Invasive Properties of Brucella melitensis 16M Outer Membrane Protein Vaccine Candidates Identified via a Reverse Vaccinology Approach. PLOS ONE. 2013;8(3):e59751.
• Chen H., et al. In Vivo Study of Spherical Gold Nanoparticles: Inflammatory Effects and Distribution in Mice. PLOS ONE. 2013;8(2):e58208.
• Moshiri A., et al. Role of Tissue-Engineered Artificial Tendon in Healing of a Large Achilles Tendon Defect Model in Rabbits. J Am Coll Surg. 2013 Jun 29. pii: S1072-7515(13)00312-8.
• Moshiri A., et al. Effectiveness of hybridized nano- and microstructure biodegradable, biocompatible, collagen-based, three-dimensional bioimplants in repair of a large tendon-defect model in rabbits. J Tissue Eng Regen Med. 2013 May 2. doi: 10.1002/term.1740.
• Chellan B., et al. LIGHT/TNFSR14 Can Regulate Hepatic Lipase Expression by Hepatocytes Independent of T Cells and Kupffer Cells. PLoS One. 2013;8(1):e54719.
• Hao J., et al. rFliC prolongs allograft survival in association with the activation of recipient Tregs in a TLR5-dependent manner.Cell Mol Immunol. 2013 Oct 7. doi: 10.1038/cmi.2013.44.
• Sadraeian M., et al. Prevention and Inhibition of TC-1 Cell Growth in Tumor Bearing Mice by HPV16 E7 Protein in Fusion with Shiga Toxin B-Subunit from Shigella dysenteriae. Cell J. 2013 Jul;15(2):176-81.
• Tripathi A., et al. Modulation of the CXC Chemokine Receptor 4 Agonist Activity of Ubiquitin through C-Terminal Protein Modification. Biochemistry. 2013 Jun 18;52(24):4184-92.
• Kovacs-Nolan J., et al. β-1,4-mannobiose stimulates innate immune responses and induces TLR4-dependent activation of mouse macrophages but reduces severity of inflammation during endotoxemia in mice. J Nutr. 2013 Mar;143(3):384-91.
• Schepetkin IA., et al. Immunomodulatory and hemagglutinating activities of acidic polysaccharides isolated from Combretum racemosum. Int Immunopharmacol. 2013 Mar;15(3):628-37.
• Ambalavanan N., et al. Titanium oxide nanoparticle instillation induces inflammation and inhibits lung development in mice. Am J Physiol Lung Cell Mol Physiol. 2013 Feb 1;304(3):L152-61.
• Mishra B., et al. A novel antimicrobial peptide derived from modified N-terminal domain of bovine lactoferrin: design, synthesis, activity against multidrug -resistant bacteria and Candida. Biochim Biophys Acta. 2013 Feb;1828(2):677-86.
• A Mountney, et al. Sialidase, Chondroitinase Abc And Combination Therapy After Spinal Cord Contusion Injury. J Neurotrauma. 2013 Feb 1;30(3):181-90.
• Bastiaan-Net S., et al. Biochemical And Functional Characterization Of Recombinant Fungal Immunomodulatory Proteins (Rfips). Int Immunopharmacol. 2013 Jan;15(1):167-75.
• Sameera Sayeed, et al. Multifunctional Role Of Human Splunc1 In Pseudomonas Aeruginosa Infection. Infect Immun. 2013 Jan;81(1):285-91.
• K Kouakou, et al. Immunomodulatory activity of polysaccharides isolated from Alchornea cordifolia. J Ethnopharmacol. 2013 Mar 7;146(1):232-42.
• Kaliannan K., et al. Intestinal alkaline phosphatase prevents metabolic syndrome in mice. Proc Natl Acad Sci USA. 2013 Apr 23;110(17):7003-8.
• Gomez G., et al. Immunogenic and Invasive Properties of Brucella melitensis 16M Outer Membrane Protein Vaccine Candidates Identified via a Reverse Vaccinology Approach. PLOS ONE. 2013;8(3):e59751.
• Chen H., et al. In Vivo Study of Spherical Gold Nanoparticles: Inflammatory Effects and Distribution in Mice. PLOS ONE. 2013;8(2):e58208.
• Moshiri A., et al. Role of Tissue-Engineered Artificial Tendon in Healing of a Large Achilles Tendon Defect Model in Rabbits. J Am Coll Surg. 2013 Jun 29. pii: S1072-7515(13)00312-8.
• Moshiri A., et al. Effectiveness of hybridized nano- and microstructure biodegradable, biocompatible, collagen-based, three-dimensional bioimplants in repair of a large tendon-defect model in rabbits. J Tissue Eng Regen Med. 2013 May 2. doi: 10.1002/term.1740.
• Chellan B., et al. LIGHT/TNFSR14 Can Regulate Hepatic Lipase Expression by Hepatocytes Independent of T Cells and Kupffer Cells. PLoS One. 2013;8(1):e54719.
• Hao J., et al. rFliC prolongs allograft survival in association with the activation of recipient Tregs in a TLR5-dependent manner.Cell Mol Immunol. 2013 Oct 7. doi: 10.1038/cmi.2013.44.
• Sadraeian M., et al. Prevention and Inhibition of TC-1 Cell Growth in Tumor Bearing Mice by HPV16 E7 Protein in Fusion with Shiga Toxin B-Subunit from Shigella dysenteriae. Cell J. 2013 Jul;15(2):176-81.
• Tripathi A., et al. Modulation of the CXC Chemokine Receptor 4 Agonist Activity of Ubiquitin through C-Terminal Protein Modification. Biochemistry. 2013 Jun 18;52(24):4184-92.